Histological Evaluation of Decellularized Skeletal Muscle Tissue Using Two Different Decellularization Agents
نویسندگان
چکیده
منابع مشابه
Histological Evaluation of Decellularized Skeletal Muscle Tissue Using Two Different Decellularization Agents.
The aim of the present study was to determine effect of two decellularized agents, sodium dodecyl sulphate (SDS) and Triton X-100, to the skeletal muscle tissue. Final scaffold was evaluated by several histological techniques to analyse preservation of essential structures including collagen and elastic fibres, basement membranes, glycosaminoglycans and also to confirm elimination of nuclear an...
متن کاملEvaluation of decellularized extracellular matrix of skeletal muscle for tissue engineering.
OBJECTIVE We evaluated the effectiveness of enzyme-detergent methods on cell removal of mouse skeletal muscle tissue and assessed the biocompatibility of the decellularized tissues by an animal model. METHODS The mouse latissimus dorsi (LD) muscles underwent decellularization with different enzyme-detergent mixtures (trypsin-Triton X-100, trypsin-sodium dodecyl sulfate (SDS), trypsin-Triton X...
متن کاملEffects of two different decellularization routes on the mechanical properties of decellularized lungs
Considering the limited number of available lung donors, lung bioengineering using whole lung scaffolds has been proposed as an alternative approach to obtain lungs suitable for transplantation. However, some decellularization protocols can cause alterations on the structure, composition, or mechanical properties of the lung extracellular matrix. Therefore, the aim of this study was to compare ...
متن کاملNear infrared spectroscopy for evaluation of skeletal muscle tissue oxygenation in different types of shock
Clinical examination is non-invasive, but has well-recognized limitations in detecting compensated and uncompensated low flow states and their severity. This paper describes the principles of near infra-red spectroscopy (NIRS) and the basis for its proposed use, in hypovolaemic, cardiogenic and septic shock, for assessing global and regional tissue oxygenation. The vascular occlusion test is ex...
متن کاملDecellularized Human Skeletal Muscle as Biologic Scaffold for Reconstructive Surgery
Engineered skeletal muscle tissues have been proposed as potential solutions for volumetric muscle losses, and biologic scaffolds have been obtained by decellularization of animal skeletal muscles. The aim of the present work was to analyse the characteristics of a biologic scaffold obtained by decellularization of human skeletal muscles (also through comparison with rats and rabbits) and to ev...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Acta Medica (Hradec Kralove, Czech Republic)
سال: 2016
ISSN: 1211-4286,1805-9694
DOI: 10.14712/18059694.2017.36